Connections of leptin and leptin receptors play crucial functions during animal development and regulation of appetite and energy balance. in situ hybridization cRNA probes, RT-PCR was performed using zebrafish specific primers (forward primer 1, 5- GGTCTCACTGCCTGTCCATT-3; reverse primer 1, 5-AGATGGTGCTGCTCCACT-3) and total RNA from zebrafish 20C50 hpf embryos. The producing DNA fragment, corresponding to nucleotides… Continue reading Connections of leptin and leptin receptors play crucial functions during animal