A technique using ‘tritiated thymidine suicide’ has been established as a means of studying the response to cytotoxic medicines of cells at different depths within multicellular tumour spheroids. cell lung malignancy cell collection, POC, have been KW-6002 manufacturer used. Using clonogenic assay, the response of these two cell types to adriamycin (ADM), nitrogen mustard (HN2), CCNU and vincristine (VCR) (POC only) were measured. The preliminary part of this study has confirmed the Rabbit polyclonal to alpha Actin cells killed are those which incorporate 3HTdR during the DNA synthesis period; the cells killed are primarily located in the outer regions of spheroids i.e. surviving cells are KW-6002 manufacturer mostly located in the inner part of the viable rim and 3HTdR pretreatment does not sensitise surviving cells to subsequent cytotoxic drug treatment. Results from large EMT6 spheroids agree with our previous findings KW-6002 manufacturer (obtained using a selective disaggregation method) that cells in the outer regions of spheroids are more sensitive to KW-6002 manufacturer ADM and HN2 than cells in the inner regions whilst the opposite is true for CCNU. For POC spheroids, cells in the outer region of spheroids are more sensitive KW-6002 manufacturer to ADM and VCR than cells in the inner region whilst a reverse trend is seen for the response to CCNU. The response to HN2 is similar whatsoever depths. Amongst the factors governing the response of cells in spheroids to cytotoxic medicines, the reactions to ADM and VCR are thought to be mainly dictated by cell cycle distribution and limited drug penetrability, whilst for HN2 the response may be determined by the element of cell cycle distribution. For CCNU, we believe that the cellular response is largely dependent upon microenvironmental factors prevailing within spheroids. Full text Full text is available like a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (1.4M), or click on a page image below to browse page by page. Links to PubMed will also be available for Selected Referrals.? 367 368 369 370 371 372 373 374 ? Selected.
