Aim: Desire to was to investigate the antiallergic effect of an aqueous leaf extract of (ALPS) in a murine model of ovalbumin (OVA)-induced allergic conjunctivitis (AC). conjunctival stroma (measured by an inflammatory score) in histopathological studies was also significantly low ( 0.05C0.01) on pretreatment. Conclusion: The ALPS exhibited interesting antiallergic activity and hence could be useful in managing AC. Linn was found in its topical use as an ocular anodyne in Gambia. The antiinflammatory effect and safety of this plant’s extract in the management of uveitis has been exhibited.[9,10] In addition, is already included in herbal preparations for the management of asthma; an allergic disorder of the respiratory system.[11] It is on this premise that this antiallergic effect of an aqueous extract of (ALPS) was investigated to determine its potential in the therapeutic management of AC. MATERIALS AND METHODS Herb collection and authentication Pistia stratiotes was collected from the Fosu lagoon, in the Central Region of Ghana, in December 2010, and authenticated in the Department of Herbal Medicine, KNUST, Kumasi, Ghana where a voucher specimen (KNUST/HM1/11/W002) has been deposited. Preparation of aqueous leaf extract of were washed, air-dried, and powdered using a hammer mill. A 700 g quantity of the powder was soaked within a liter of drinking water for 24 h. Reflux purification was performed at 80C. The filtrate was freeze-dried using a Hull freeze-dryer/lyophilizer 140 SQ Foot (model 140FS275C; CUDC-101 Hull, Warminster, PA), tagged ALPS, and kept at 4oC (produce 4.7%). Phytochemical testing of aqueous leaf remove of was screened pursuing recommended protocols defined for the current presence of phytochemicals by Trease and Evans.[12] Ethical and biosafety factors The scholarly research protocols had been accepted by the Departmental Ethics Committee. All actions performed through the research conformed to recognized principles for lab animal make use of and treatment (European union directive of 1986: 86/609/EEC). Biosafety suggestions for security CUDC-101 of personnel within the lab were observed. Medications and chemical substances Ovalbumin (OVA) (Cayla-InvivoGen, Toulouse, France), Lightweight aluminum hydroxide (Hopkins and Williams Limited, Chadwell Heath, Essex, UK), chloroform (VWR International Ltd, Leicester, UK), and formalin (Yash Chemical substances, India) had been some chemicals found in this research. Experimental pets Eight-week outdated Imprinting Control Area (ICR) mice of either sex weighing 18-24 g had been provided by the pet House Unit from the Section of Pharmacology, KNUST, Kumasi, Ghana. These pets were held in CUDC-101 metallic cages under ambient circumstances of temperatures (26 3C), comparative Rabbit Polyclonal to HS1 (phospho-Tyr378). dampness (60-70%) and light/dark cycles. Mice received normal industrial mice chow pellet from Agricare Limited, Kumasi, Ghana, and drinking water = 7). Groupings ICV had been treated with either 2 ml/kg regular saline (NS), 5 mg/kg cetirizine (CET), or 10, 50 or 100 mg/kg ALPS respectively, 1 h before OVA problem. Group VI had not been challenged. A standard control Group (VII) was also held under experimental circumstances. Conjunctival redness, cover edema, and tearing had been noticed under a SL500 Shin Nippon Slit Light fixture (Ajinomoto Trading Inc., Tokyo, Japan), had been scored on the range of 0-3 30 min following the last topical ointment challenge.[14] Cover scratching was monitored for 30 s, as well as the frequency of scratching was counted. Only 1 eye of every animal was evaluated and data provided as the indicate per group. Ovalbumin-specific antibodies assay Mice had been anesthetized with chloroform CUDC-101 and bloodstream gathered by cardiac puncture into Eppendorf pipes (Sigma-Aldrich, St. Louis, MO, USA) and permitted to clot. The clotted bloodstream was centrifuged (temperatures 25C, swiftness 3000 g) for 5 min utilizing a.