Supplementary MaterialsFigure 1source data 1: Overview table of segmentation clock tissue and cellular oscillatory properties. treated with Fgf8b (= 547), using multiple donor embryos in each of 4 impartial experimental replicates (= 4), carried out on separate days. Across the 29 fields recorded, we observed cell divisions in both YFP-negative (30, 5% of total cells) and YFP-positive cells (13, 2% of total cells). We found a range in the number of cell divisions from 0 to 5 cells per field, with an average of 1.5 (1 SD) divisions per field. The categories of disqualification list the event in a recording that led to disqualification. For example, four divisions in YFP-positive cells occurred after the cell had been disqualified for another reason (movement in and out of Aminothiazole field, touching another cell).DOI: http://dx.doi.org/10.7554/eLife.08438.005 elife-08438-fig1-data2.docx (91K) DOI:?10.7554/eLife.08438.005 Figure 1source data 3: Time series data from low-density segmentation clock cells. XLS file containing all time series data for each of the 147 low-density segmentation clock cells in the presence of Fgfb. The file contains 4 work-sheets corresponding to each of the 4 impartial replicates and to the plots in Physique 1figure supplement 5. In each sheet, each cell is usually described by 3 neighboring columns: average fluorescence, local background, and background subtracted signal. Cells are also listed by their field Cxcr2 of view in the original microscopy files.DOI: http://dx.doi.org/10.7554/eLife.08438.006 elife-08438-fig1-data3.xlsx (1.5M) DOI:?10.7554/eLife.08438.006 Figure 3source data 1: Aminothiazole Precision and period calculation for persistent segmentation clock oscillators. Each set of panels shows, successively, the background-subtracted average YFP intensity levels over time from a single persistently oscillating cell in black; the cosine of the phase calculated from the wavelet transformation in blue; and the autocorrelation Aminothiazole function in green. The dashed green curve shows the analytical fit of the autocorrelation. Both period and quality factor can be calculated from this procedure (see Supplementary document 1). This is actually the complete continual cell data established, a sub-set from the low-density established, that the plots of period andquality element in Figure D and 3B are generated.DOI: http://dx.doi.org/10.7554/eLife.08438.023 elife-08438-fig3-data1.pdf (1.4M) DOI:?10.7554/eLife.08438.023 Body 3source data 2: Accuracy and period calculation for the tissue-level segmentation clock in the zebrafish embryo. For data established health supplement 3C1, each group of sections displays, successively, the background-subtracted typical YFP intensity amounts from an area of posterior PSM tissues within a embryo in dark; the cosine from the stage calculated through the wavelet change in blue; as well as the autocorrelation function in green. The dashed green curve displays the analytical in shape from the autocorrelation. Both quality and period factor could be calculated out of this procedure. The original strength versus period data originates from Soroldoni et al. (2014). This is actually the full dataset from time-lapse data of 24 embryos that the story of quality element in Body 3B is certainly generated.DOI: Aminothiazole http://dx.doi.org/10.7554/eLife.08438.024 elife-08438-fig3-data2.pdf (1.4M) DOI:?10.7554/eLife.08438.024 Body 3source data 3: Accuracy and period calculation for persistent circadian clock oscillators. For data established health supplement 3C1, each group of sections displays, successively, the background-subtracted intensity levels from an individual oscillating Per2-Lucifcerase-expressing fibroblast as time passes in dark persistently; the cosine from the stage calculated through the wavelet change in blue; as well as the autocorrelation function in green. The dashed green curve displays the analytical in shape from the autocorrelation. Both period and quality aspect can be computed from this treatment. The.